Clonal propagation of Wattakaka volubilis through nodal explant culture

Abstract

A protocol was developed for microporpagation of Wattakaka volubils (L.f) Stapf, an important medicinal plant in India. In vitro shoot multiplication nodal explants of W. volubilis was achieved on solid MS basal medium supplemented with BAP (6-Benzylaminopurine) and NAA (Naphthalene acetic acid). Presence of NAA in the culture medium along with BAP promoted multiplication of shoots than BAP alone. The highest shoot multiplication rate (23.4±0.48) was observed after 28 days of culture on MS basal medium supplemented with 0.6 mg/l BAP and 0.2 mg/l NAA. Elongated shoots produced roots within 6 weeks in half strength MS basal medium supplemented with 0.6 mg/l IBA (Indole-3-butyric acid) and 3% (w/v) sucrose. Rooted plantlets were acclimatized with a survival rate of 95% and transferred to the green-house and thereafter successfully transferred to the field with 90% survival rate.  

Keywords: medicinal plants, micropropagation, nodal culture, survival rate

DOI: http://dx.doi.org/10.4038/cjsbs.v40i1.3406

CJSBS 2011; 40(1): 53-58